High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength ModelGradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well known authorities in liquid chromatography, High Performance Gradient Elution: